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| Catalog Number | orb2383 |
|---|---|
| Category | Antibodies |
| Description | VGLL3 Rabbit Polyclonal Antibody |
| Target | VGLL3 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Human, Porcine, Rabbit, Rat, Sheep |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Purification | Affinity purified by Protein A |
| Immunogen | KLH conjugated synthetic peptide derived from human VGLL3 (85-190/326aa) |
| UniProt ID | A8MV65 |
| MW | 36 kDa |
| Tested applications | IF, IHC-Fr, IHC-P |
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Colon carcinoma related protein; Vestigial like 3( Read more... |
| Research Area | Developmental Biology |
| Note | For research use only |

Paraformaldehyde-fixed, paraffin embedded (Mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (VGLL3) Polyclonal Antibody, Unconjugated (orb2383) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
WB | |
Canine, Equine, Guinea pig, Mouse, Porcine, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-Fr, IHC-P | |
Bovine, Canine, Equine, Human, Porcine, Rabbit, Rat, Sheep | |
Mouse | |
Rabbit | |
Polyclonal | |
HRP |
IF | |
Bovine, Canine, Equine, Human, Porcine, Rabbit, Rat, Sheep | |
Mouse | |
Rabbit | |
Polyclonal | |
APC |
IF | |
Bovine, Canine, Equine, Human, Porcine, Rabbit, Rat, Sheep | |
Mouse | |
Rabbit | |
Polyclonal | |
FITC |
IF, IHC-Fr, IHC-P | |
Bovine, Canine, Equine, Human, Porcine, Rabbit, Rat, Sheep | |
Mouse | |
Rabbit | |
Polyclonal | |
Biotin |
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