VEGFR2/KDR/CD309 Antibody

• Catalog Number: orb1294363
• Category: Antibodies
• Description: Rabbit polyclonal antibody to VEGF Receptor 2
• Target: VEGFR2 / KDR / CD309
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human VEGF Receptor 2 / KDR
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500 – 1:1000 Immunofluorescence 1:100 – 1:300 Immunohistochemistry (Paraffin) 1:50 – 1:200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cardiovascular Research
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294363 anti-VEGF Receptor 2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VEGF Receptor 2 with orb1294363 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VEGF Receptor 2 with orb1294363 at 1/200.

VEGF Receptor 2 antibody - at 1/500 dilution. Lysates at 50 µg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294363 overnight at 4°C.

VEGF Receptor 2 antibody - at 1/500 dilution. Lysates at 50 µg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294363 overnight at 4°C.