VEGFR2 Rabbit Polyclonal Antibody

SKU: orb1294363

Description

Rabbit polyclonal antibody to VEGF Receptor 2

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:500 – 1:1000, Immunofluorescence 1:100 – 1:300, Immunohistochemistry (Paraffin) 1:50 – 1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human VEGF Receptor 2 / KDR
• Target: VEGFR2 / KDR / CD309
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294363 anti-VEGF Receptor 2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VEGF Receptor 2 with orb1294363 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VEGF Receptor 2 with orb1294363 at 1/200.

VEGF Receptor 2 antibody - at 1/500 dilution. Lysates at 50 µg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294363 overnight at 4°C.

VEGF Receptor 2 antibody - at 1/500 dilution. Lysates at 50 µg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294363 overnight at 4°C.

Quick Database Links

Gene Symbol

VEGFR2 / KDR / CD309