VEGFR1 Rabbit Polyclonal Antibody

• Catalog Number: orb11556
• Category: Antibodies
• Description: VEGFR1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Equine, Porcine
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse VEGFR1 (1162-1260/1333aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 147 kDa
• Target: FLT1
• RRID: AB_10748198
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Vascular endothelial growth factor receptor 1; VEG
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Graham, N. et al. Placental expression of anti-angiogenic proteins in mirror syndrome: a case report Placenta, 33, 528-531 (2012)

PubMed: 22401877

Applications

IHC

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (VEGFR1) Polyclonal Antibody, Unconjugated (orb11556) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Recombinant human VEGFR1 protein, C-His (HEK293), Primary: Anti-VEGFR1 (orb11556) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 147 kDa, Observed band size: 140 kDa.

Tissue/Cell: 293T cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (VEGFR1) polyclonal Antibody, Unconjugated (orb11556) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.