VEGFA Rabbit Polyclonal Antibody

SKU: orb500651

Description

VEGFA Rabbit Polyclonal Antibody

Research Area

Angiogenesis, Angiogenesis and Vasculogenesis, Angiogenic factors, Cancer Research, Cell Biology, Growth Factors, Growth Factors and Hormones, Hypoxia, SARS /COVID-19, Varscular, Varscular System

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Canine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human VEGFA (101-200/232aa)
• Target: VEGFA
• Molecular Weight: 24 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; VEGF-A; Vascular permeability factor(VPF);

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (VEGFA) Polyclonal Antibody, Unconjugated (orb500651) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (VEGFA) Polyclonal Antibody, Unconjugated (orb500651) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebellum (Rat) Lysate at 40 ug, Kidney (Rat) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Liver (Rat) Lysate at 40 ug, Primary: Anti-VEGFA (orb500651) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43/23 kD, Observed band size: 23 kD.

Sample: Lane 1: Human K562 cell lysates, Lane 2: Human MDA-MB-231 cell lysates, Primary: Anti-VEGFA (orb500651) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kDa, Observed band size: 24 kDa.

Quick Database Links

Gene Symbol

VEGFA

UniProt

P15692