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Description
Research Area
Cardiovascular Research; Cell Biology
Images & Validation
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Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 at 2 ug/mL (100 uL/well) can bind Rhesus macaque VEGF R2, His Tag with a linear range of 4-60 ng/mL. |
| Target | VEGF R2 |
| Tag | His Tag |
| MW | 85.2 kDa |
| Purity | >97% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Ala 20 - Glu 764 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−KDR,CD309,FLK1,VEGFR,VEGFR2
Similar Products
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VEGF R2 Recombinant Protein [orb1228472]
Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
110.4 kD
Human Cells
0.05 mg
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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