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Catalog Number | orb80646 |
---|---|
Category | Proteins |
Description | Recombinant of VEGF E protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | The protein was lyophilized from a concentrated (1mg/ml) solution containing PBS. |
Purity | Greater than 90.0% as determined by SDS-PAGE. |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH DSTKTWSEVF ENSGCKPRPM VFRVHDEHPE LTSQRFNPPC VTLMRCGGCC NDESLECVPT EEANVTMQLM GASVSGGNGM QHLSFVEHKK CDCKPPLTTT PPTTTRPPRR RR |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Biological Activity | The biological activity was determined (1) by the ability to induce VEGFR-2/KDR receptor phosphorylation in PAE/KDR cells and (2) in a cell proliferation assay using primary HUVECs. The ED50 for this effect is typically 1-5ng/ml. |
Solubility (25°C) | The lyophilized oVEGF-E Orf Virus should be reconstituted in water or medium to a concentration not lower than 50µg/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin. |
Storage | Stability: Lyophilized Vascular Endothelial Growth Factor-E Orf Virus although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF E -OV should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles |
Note | For research use only |
IF, IHC-Fr, IHC-P | |
Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
Human | |
6.25 pg/mL-400 pg/mL | |
1.56 pg/mL |