VE-Cadherin Antibody

• Catalog Number: orb1566960
• Category: Antibodies
• Description: VE-Cadherin Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Mouse
• Immunogen: residues 25-595 (ECD)
• Concentration: 500 µg/ml
• Dilution range: WB - 1:1,000; IP - 12 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: VE-Cadherin
• UniProt ID: P55284
• NCBI: NP_033998.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: cadherin-5; VECD; VE-cadherin; VE-Cad; VEcad; Vec;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse VE-Cadherin by western blot. Samples: Whole cell lysate (2 µg) from NIH 3T3, CT26, bEnd.3, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VE-Cadherin recombinant monoclonal antibody (orb1566960) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.