VE-cadherin/CDH5/CD144 Antibody

• Catalog Number: orb1973151
• Category: Antibodies
• Description: VE-cadherin/CDH5/CD144 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Immunogen: cadherin 5, type 2 (vascular endothelium)
• Dilution range: Western Blot 1:1000 Immunofluorescence 1:300 – 1:500 Immunohistochemistry (Paraffin) 1:100 – 1:200
• Conjugation: Unconjugated
• Target: VE-cadherin / CDH5 / CD144
• Storage: Long time storage is recommended at -20°C. 100mM Tris Glycine, 1% BSA, 30% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1973151 overnight at 4°C. Anti VE-Cadherin/CD144 antibody at 1/1000 dilution, Lysates at 35 μg per lane.

Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1973151 anti VE-Cadherin antibody (green) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Hepatocellular carcinoma (HCC) and human placenta tissue labeling VECadherin/CD144 antibody with orb1973151 at 1/150.

Immunohistochemical analysis of paraffin embedded human placenta tissue labeling VECadherin/CD144 antibody with orb1973151 at 1/150.

Immunohistochemical analysis of paraffin embedded Hepatocellular carcinoma (HCC) tissue labeling VE-Cadherin/CD144 antibody with orb1973151 at 1/150.