VDAC3 Rabbit Polyclonal Antibody

• Catalog Number: orb101384
• Category: Antibodies
• Description: VDAC3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit, Sheep
• Reactivity: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human VDAC3 (101-200/283aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 31 kDa
• Target: VDAC3
• UniProt ID: Q9Y277
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: hVDAC3; mVDAC3; Outer mitochondrial membrane prote
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Wei Meng 1, Linghua Li N6-methyladenosine modification of SPOP relieves ferroptosis and diabetic cardiomyopathy by enhancing ubiquitination of VDAC3 Free Radic Biol Med, (2024)

PubMed: 39549880

Applications

CoIP, IF

Reactivity

Mouse

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Rat Kidney tissue lysates, Lane 4: Rat Spleen tissue lysates, Primary: Anti-VDAC3 (orb101384) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kDa, Observed band size: 31 kDa.

Sample: Large intestine (Rat) Lysate at 40 ug, Primary: Anti-VDAC3 (orb101384) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Tissue/cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-VDAC3 Polyclonal Antibody, Unconjugated (orb101384) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.