VCP Antibody

• Catalog Number: orb259631
• Category: Antibodies
• Description: VCP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human VCP (732-760aa RRDHFEEAMRFARRSVSDNDIRKYEMFAQ), identical to the related rat and mouse sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 89322 MW
• UniProt ID: P55072
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Transitional endoplasmic reticulum ATPase;TER ATPa
• Note: For research use only
• Application notes: WB: The detection limit for VCP is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-VCP antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB:1:HeLa;2:A431;3:U-87MG;4:A549;5:SH-SY5Y cell;6:K562 cell;7:Raji cell;8:HepG2 cell;9:rat heart tissue;10:rat spleen tissue;11:rat kidney tissue;12:rat liver tissue;13:mouse heart tissue;14:mouse kidney tissue;15:mouse liver tissue;16:HEPA1-6 cell.

IF analysis of VCP using anti-VCP antibody. VCP was detected in immunocytochemical section of A431 cells.

IHC analysis of VCP using anti-VCP antibody. VCP was detected in paraffin-embedded section of human glioma tissue.

IHC analysis of VCP using anti-VCP antibody. VCP was detected in paraffin-embedded section of human glioma tissue.

IHC analysis of VCP using anti-VCP antibody. VCP was detected in paraffin-embedded section of human meningeoma tissue.

IHC analysis of VCP using anti-VCP antibody. VCP was detected in paraffin-embedded section of mouse brain tissue.

IHC analysis of VCP using anti-VCP antibody. VCP was detected in paraffin-embedded section of rat brain tissue.

IHC analysis of VCP using anti-VCP antibody. VCP was detected in frozen section of mouse brain tissue.

IHC analysis of VCP using anti-VCP antibody. VCP was detected in frozen section of rat brain tissue.