VCL Antibody

• Catalog Number: orb1238146
• Category: Antibodies
• Description: VCL Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: [7E10]
• Tested applications: ELISA, WB
• Predicted Reactivity: Porcine
• Reactivity: Gallus, Human, Mouse, Rabbit, Rat
• Isotype: IgG
• Immunogen: Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.The immunogen is located within amino acids 50 - 100 of Vinculin.
• Concentration: 1 mg/mL
• Dilution range: Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 117 kDa Observed: 120 kDa
• Target: VCL
• NCBI: NP_003364
• Storage: Vinculin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: CMD1W, CMH15, Metavinculin, MVCL
• Note: For research use only
• Application notes: Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Vinculin in 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and rat heart, mouse lung, rat lung, mouse spleen, rat spleen, rabbit spleen, mouse brain, rabbit brain and chicken spleen tissue lysate with Vinculin antibody at 1 μg/ml.