VCAM-1 Rabbit Polyclonal Antibody

SKU: orb1294364

Description

Rabbit polyclonal antibody to VCAM-1

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:500–1:1000, Immunofluorescence 1:200–1:500, Immunohistochemistry (Paraffin) 1:100–1:200
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human VCAM-1 / CD106
• Target: VCAM-1 / CD106 (Extracellular)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294364 anti-VCAM-1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling VCAM-1 / CD106 with orb1294364 at 1/100.

Immunohistochemical analysis of paraffin embedded Human colorectal cancer tissue labeling VCAM-1 / CD106 with orb1294364 at 1/100.

Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling VCAM-1 / CD106 with orb1294364 at 1/100.

VCAM-1 / CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4°C.

VCAM-1 / CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4°C.

VCAM-1 / CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4°C.

Quick Database Links

Gene Symbol

VCAM-1 / CD106 (Extracellular)