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Item 1 of 7
Item 1 of 7
VCAM-1 / CD106 (Extracellular)
Catalog Number: orb1294364
| Catalog Number | orb1294364 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to VCAM-1 |
| Target | VCAM-1 / CD106 (Extracellular) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit-IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human VCAM-1 / CD106 |
| Tested applications | IF, IHC, WB |
| Dilution range | Western Blot 1:500–1:1000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100–1:200 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Note | For research use only |
VCAM-1/CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4°C.
VCAM-1/CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294364 Anti-VCAM-1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling VCAM-1/CD106 with orb1294364 at 1/100.
Immunohistochemical analysis of paraffin embedded Human colorectal cancer tissue labeling VCAM-1/CD106 with orb1294364 at 1/100.
VCAM-1/CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling VCAM-1/CD106 with orb1294364 at 1/100.
