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Catalog Number | orb763375 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to V5-Tag |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | IF, IP, WB |
Isotype | IgG1 |
Immunogen | Synthetic Peptide |
Concentration | 1mg/ml |
Dilution range | WB 1:5,000 IP 1:200 IF 1:1,000 |
Conjugation | Unconjugated |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IP antibody use: 5ug V5 Mouse IgG1 per ml Lysate, WB 1:5000 1: untransfected 293 cell lysate 2: transfected 293 cell lysate with V5-tag fusion protein 3: IP (untransfted 293 + anti-V5 mAb + Protein G agarose) 4: IP (transfected 293 + normal Mouse IgG + Protein G ag).
IF analysis of 293 cells transfected with a V5-tag protein, using ELKbio anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5).
1ug V5 fusion protein + Primary antibody dilution at 1: 1:5, 000 2: 1:10, 000.
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