V5 Tag Antibody

• Catalog Number: orb1530832
• Category: Antibodies
• Description: V5 Tag Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IP, WB
• Immunogen: V5 Epitope tag (GKPIPNPLLGLDST)
• Concentration: 250 µg/ml
• Dilution range: WB - 1:1,000; IP - 20 µl/1 mg lysate; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: V5 Tag
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of V5-tagged protein by WB from HEK293 transfected with Met-V5-IDO1, HEK293 with IDO1-V5-HA (Internal Tag), and HEK293 with IDO1-HA-V5 (C-terminal Tag) cells, using Rabbit anti-V5 Tag recombinant monoclonal antibody.

Detection of V5 Tag by western blot of immunoprecipitates from HEK293 transfected with IDO1-V5.Antibodies: Antibodies: Rabbit anti-V5 recombinant monoclonal antibody.

Detection of V5 tag in FFPE HEK293 cells expressing V5 tagged nuclear protein by ICC.Antibody: Rabbit anti-V5 tag recombinant monoclonal antibody.

Detection of V5 tag in FFPE HEK293 cells expressing V5 tagged cytoplasmic protein by ICC.Antibody: Rabbit anti-V5 tag recombinant monoclonal antibody.