USP25 antibody

• Catalog Number: orb341409
• Category: Antibodies
• Description: Rabbit polyclonal antibody to USP25
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human USP25
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: USP25
• Entrez: 30940, 29761
• UniProt ID: P57080, Q9UHP3
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: USP21; Ubiquitin carboxyl-terminal hydrolase 25; D
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of USP25 expression in SKOV3 (A), PC12 (B), mouse brain (C), mouse liver (D) whole cell lysates. (Predicted band size: 122; 125; 129 kD; Observed band size: 130 kD)

Immunohistochemical analysis of USP25 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.