Urokinase Rabbit Polyclonal Antibody

• Catalog Number: orb11278
• Category: Antibodies
• Description: Urokinase Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PLAU (361-432/432aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 49 kDa
• Target: PLAU
• UniProt ID: P00749
• RRID: AB_10748947
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Urokinase; ATF; ATF uPA; PLAU; UPA; Plasminogen ac
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhang, Bo et al. UPA-sensitive ACPP-conjugated nanoparticles for multi-targeting therapy of brain glioma Biomaterials, 36, 98-109 (2015)

PubMed: 25443789

Sample: Cerebrum (mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Urokinase (orb11278) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49kD, Observed band size: 48kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Urokinase (orb11278) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 49 kD.

Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Lung (Mouse) Lysate at 40 ug, Lane 4: Spleen (Mouse) Lysate at 40 ug, Lane 5: Liver (Rat) Lysate at 40 ug, Lane 6: Lung (Rat) Lysate at 40 ug, Lane 7: Spleen (Rat) Lysate at 40 ug, Primary: Anti-Urokinase (orb11278) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40-80 kD, Observed band size: 45 kD.