UNC5C Antibody

• Catalog Number: orb1249537
• Category: Antibodies
• Description: UNC5C Antibody
• Target: UNC5C
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: NCTVSEEPTGID
• UniProt ID: O95185
• Tested applications: ELISA, IF, IHC
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in Human and Mouse Brain lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows staining of some neuronal bodies plus intense axonal staining. Recommended concentration: 3-5ug/ml.Immunofluorescence: Staining of paraffin embedded endocrine islets (red) in adult Mouse Pancreas. Nuclear staining in blue and ductal cell staining in green. Data obtained from a customer.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: UNC5C, unc-5 homolog C (C. elegans), UNC5H3, homol
• Research Area: Neuroscience
• Note: For research use only
• NCBI: NP_003719.2

orb1249537 (50 ug/ml) staining of islets in Mouse Pancreas. Citrate antigen retrieval. Detected by immunofluorescence.

orb1249537 (3.8 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.