UNC13D Antibody

• Catalog Number: orb1246812
• Category: Antibodies
• Description: UNC13D Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-ETQKHKKDLHPLFD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx. 105kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123kDa according to NP_954712.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Thymus shows cytoplasm staining in select areas. Recommended concentration: 2.5ug/ml. In paraffin embedded Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out. Data kindly provided by Micheal Tuvim, University of Texas MD Anderson Cancer Center, Houston, USA. Recommended concentration: 1-2ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: UNC13D
• UniProt ID: Q70J99
• NCBI: NP_954712.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: UNC13D, Munc13-4, unc-13 homolog D (C. elegans) ,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx. 105kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123kDa according to NP_954712.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Thymus shows cytoplasm staining in select areas. Recommended concentration: 2.5ug/ml. In paraffin embedded Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out. Data kindly provided by Micheal Tuvim, University of Texas MD Anderson Cancer Center, Houston, USA. Recommended concentration: 1-2ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246812 (2 ug/ml) staining of Human T-lymphocyte lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246812 (2.5 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246812 (0.5 ug/ml) staining of paraffin embedded Mouse Lung (wt in A and KO in B).