UCHL1/PGP9.5 Mouse Monoclonal Antibody

• Catalog Number: orb783384
• Category: Antibodies
• Description: UCHL1/PGP9.5 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: T2F5
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Monkey, Mouse, Porcine, Rat, Xenopus
• Reactivity: Human
• Isotype: IgG1
• Immunogen: KLH conjugated synthetic peptide derived from human UCHL1/PGP9.5 (151-223/223aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:20-50, IHC-F=1:400-800, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 25 kDa
• Target: UCHL1
• UniProt ID: P09936
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Gracile axonal dystrophy; Neuron cytoplasmic prote
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human astroglioma section), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with UCHL1/PGP9.5) Monoclonal Antibody, Unconjugated (orb783384) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human brain section), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with UCHL1/PGP9.5) Monoclonal Antibody, Unconjugated (orb783384) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: 293T cell lysates, Lane 2: NCI-H1299 cell lysates, Lane 3: PC-12 cell lysates, Primary: Anti-UCHL1/PGP9.5 (orb783384) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 25 kD, Observed band size: 25 kD.