UBE2Q1 Rabbit Polyclonal Antibody

• Catalog Number: orb1255
• Category: Antibodies
• Description: UBE2Q1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Bovine, Canine, Equine, Human, Rabbit, Sheep
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human UBE2Q1 (321-422/422aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=2μg /Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 46 kDa
• Target: UBE2D4
• UniProt ID: Q7Z7E8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Galactosyl transferase associated protein; GTAP; N
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhang, Bin et al. Upregulation of UBE2Q1 via gene copy number gain in hepatocellular carcinoma promotes cancer progression through β-catenin-EGFR-PI3K-Akt-mTOR signaling pathway Mol Carcinog, 57, 201-215 (2018)

PubMed: 29027712

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-UBE2Q1 antibody (orb1255), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

cells: mouse splenocyte, Concentration: 2 µg/10^6 cells, 4°C incubate 30 min.