UBE2O Antibody, KO Validated

• Catalog Number: orb1274560
• Category: Antibodies
• Description: UBE2O Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 200kDa
• Target: UBE2O
• UniProt ID: Q9C0C9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: UBE2O, ubiquitin-conjugating enzyme E2O, E2-230K,
• Note: For research use only
• Application notes: WB: 1:1000 - 1:4000IF: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using UBE2O antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody at 1:1000 dilution.

Immunofluorescence analysis of HeLa cells using UBE2O Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of A-431 cells using 3ug UBE2O antibody. Western blot was performed from the immunoprecipitate using UBE2O antibody at a dilution of 1:1000.