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UBE2J1 Antibody

SKU: orb345577

Description

UBE2J1 antibody

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA, IP, WB
Dilution RangeELISA: 1:1,000 - 1:5,000, IP: 1-2 µg, WB: 1:200 - 1:2,000
ReactivityHuman
Application Notes
This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35-40 kDa in size corresponding to Ube2j1 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.
TargetUBE2J1
PurityThis affinity purified antibody is directed against human Ube2j1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Ube2j1 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration0.71 mg/mL
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

rabbit anti-UBE2J1 antibody, rabbit anti-E2 J1 antibody, rabbit anti-Ubiquitin conjugating enzyme E2J1 antibody, Non-canonical ubiquitin-conjugating enzyme 1, Ube2j1, E2 ubiquitin-conjugating enzyme J1, NCUBE-1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E, HsUBC6e

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeq37577122

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

Select a size below

100 μg
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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