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Catalog Number | orb214710 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to UBE2A |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat, Zebrafish |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human UBE2A/B. The exact sequence is proprietary. |
Dilution range | WB: 1-500-1-1000 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | UBE2A; UBE2B |
Entrez | 81816, 22210, 7320, 22209 |
UniProt ID | P49459, Q9Z255, P63149, P63147, P63146 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti UBE2A antibody, anti RAD6A antibody, anti Ubi Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of UBE2A/B expression in MCF7 (A), A549 (B), C6 (C), MEF (D) whole cell lysates. (Predicted band size: 17 kD; Observed band size: 17 kD)
Immunofluorescent analysis of UBE2A/B staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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