UBE2A Antibody

• Catalog Number: orb1260869
• Category: Antibodies
• Description: UBE2A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 17kDa
• Target: UBE2A
• UniProt ID: P49459
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HHR6A, MRXS3MRXSN, RAD6A, UBC2, ubiquitin-conjugat
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of U2OS cells using UBE2A antibody (orb1260869) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse kidney, using UBE2A antibody (orb1260869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.