UBC Antibody

SKU: orb1257914

Description

UBC Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:1000 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBC (NP_066289.3).
• Target: UBC
• Molecular Weight: Observed: 12kD/25kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Polyubiquitin-C, Ubiquitin, UBC

Western blot analysis of extracts of various cell lines, using UBC antibody (orb1257914) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human breast carcinoma using UBC antibody (orb1257914).

Immunohistochemistry of paraffin-embedded human liver using UBC antibody (orb1257914) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using UBC antibody (orb1257914) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

UBC

UniProt

P0CG48