UBC Antibody

• Catalog Number: orb1257914
• Category: Antibodies
• Description: UBC Antibody
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBC (NP_066289.3).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 12kD/25kDa
• Target: UBC
• UniProt ID: P0CG48
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Polyubiquitin-C, Ubiquitin, UBC
• Note: For research use only
• Application notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using UBC antibody (orb1257914) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human breast carcinoma using UBC antibody (orb1257914).

Immunohistochemistry of paraffin-embedded human liver using UBC antibody (orb1257914) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using UBC antibody (orb1257914) at dilution of 1:100. Blue: DAPI for nuclear staining.