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Item 1 of 2
TYROBP Rabbit Polyclonal Antibody (FITC)
Catalog Number: orb464992
| Catalog Number | orb464992 |
|---|---|
| Category | Antibodies |
| Description | TYROBP Rabbit Polyclonal Antibody (FITC) |
| Target | TYROBP |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | FITC |
| Reactivity | Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Human, Porcine, Rat, Sheep |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide derived from human DAP12 (31-113/113aa) |
| Tested applications | FC |
| Dilution range | Flow-Cyt=1μg/Test |
| Antibody Type | Primary Antibody |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Alternative names | DAP 12; DAP12; DNAX activation protein 12; DNAX-ac Read more... |
| Note | For research use only |
Blank control (Black line): U937 (Black). Primary Antibody (green line): Rabbit Anti-DAP12 Conjugated FITC antibody (orb464992), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature). The cells were then incubated in 5% BSA to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was perf.
Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-DAP12 antibody (orb464992), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
