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Catalog Number | orb19872 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to TXNDC4 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine, Human, Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 0.2-0.6 μg/ml |
Conjugation | Unconjugated |
MW | 47 |
Target | TXNDC4 |
Entrez | 23071 |
Protein Sequence | EQKDSDNYRVFER |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti TXNDC4 antibody, anti thioredoxin domain cont Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 47kDa band observed in lysates of cell line K562 (calculated MW of 47.0kDa according to NP_055866.1). Recommended concentration: 0.2-0.6 μg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
orb19872 (0.2ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB | |
Animal, Bovine, Canine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish | |
Canine, Equine, Guinea pig, Human, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
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