Tubulin alpha antibody

• Catalog Number: orb345858
• Category: Antibodies
• Description: Tubulin alpha antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Gallus, Goat, Human, Mouse, Rat, Sheep
• Isotype: IgG
• Immunogen: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Anti-Tubulin is directed against human alpha Tubulin protein. The Tubulin Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
• Conjugation: HRP
• UniProt ID: P68363
• Storage: Store Anti-Tubulin Loading Control Antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge Loading Control if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-alpha-Tubulin Antibody HRP conjugation
• Note: For research use only
• Application notes: Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.