alpha Tubulin antibody

• Catalog Number: orb556941
• Category: Antibodies
• Description: Rabbit polyclonal antibody to alpha Tubulin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 1A. The exact sequence is proprietary.
• Concentration: 0.24 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 50
• Target: alpha Tubulin
• UniProt ID: Q71U36
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 20% Glycerol
• Alternative names: tubulin alpha 1a , B-ALPHA-1 , LIS3 , TUBA3
• Note: For research use only
• Application notes: Positive control: 293T , A431 , Jurkat , Raji , NIH-3T3
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using alpha Tubulin antibody

Immunohistochemical staining of mouse testis using alpha Tubulin antibody

Immunohistochemical staining of mouse ovar using alpha Tubulin antibody

Immunohistochemical staining of human breast cancer using alpha Tubulin antibody

Western blot analysis of 293T(Lane 1), A431(Lane 2), JurKat(Lane 3), Raji(Lane 4) using alpha Tubulin antibody

Western blot analysis of 293T(Lane 1), A431(Lane 2), JurKat(Lane 3), Raji(Lane 4) using alpha Tubulin antibody