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Catalog Number | orb344426 |
---|---|
Category | Antibodies |
Description | Tubulin alpha antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 17H11.F10 |
Tested applications | ELISA, IF, IHC, Multiplex Assay, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:300,000, IHC: 2.5 µg/mL, IF: 0.1 µg/mL, WB: 1:1,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography. This Loading Control antibody is directed against alpha tubulin. A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control. |
Conjugation | Unconjugated |
UniProt ID | P68363 |
NCBI | 17986283 |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1 Read more... |
Note | For research use only |
Application notes | Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of MCF-7 cell using Tubulin alpha antibody
Immunofluorescence analysis of MCF-7 cell using Tubulin alpha antibody
Immunofluorescence analysis of A459 cells using Tubulin alpha antibody
Immunohistochemical staining of epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs using Tubulin alpha antibody
Western blot analysis of HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 ug of lysate each using Tubulin alpha antibody
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IHC-P, IP, WB | |
Aves, Human, Invertebrate, Mouse, Paramecium, Plant, Porcine, Yeast | |
Monoclonal | |
Unconjugated |
ICC, IHC-P, WB | |
Drosophila, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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