Tubulin alpha antibody

• Catalog Number: orb344425
• Category: Antibodies
• Description: Tubulin alpha antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 17H11.F10
• Tested applications: ELISA, IF, IHC, Multiplex Assay, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:300,000, IHC: 2.5 µg/mL, IF: 0.1 µg/mL, WB: 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography. This Loading Control antibody is directed against alpha tubulin. A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
• Conjugation: Unconjugated
• UniProt ID: P68363
• NCBI: 17986283
• Storage: Store Anti-Tubulin Antibody at -20° C prior to opening. Aliquot Loading Control Antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Anti-Tubulin Loading Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1
• Note: For research use only
• Application notes: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
• Expiration Date: 12 months from date of receipt.

References

Chavez, R. D. et al. SOX9 protein is stabilized by TGF-β and regulates PAPSS2 mRNA expression in chondrocytes Osteoarthritis Cartilage, 25, 332-340 (2017)

PubMed: 27746378

Immunofluorescence analysis of MCF-7 cell using Tubulin alpha antibody

Immunofluorescence analysis of MCF-7 cell using Tubulin alpha antibody

Immunofluorescence analysis of A459 cells using Tubulin alpha antibody

Immunohistochemical staining of epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs using Tubulin alpha antibody

Western blot analysis of HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 ug of lysate each using Tubulin alpha antibody