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    Tubulin alpha antibody (Biotin)

    Catalog Number: orb345885

    DispatchUsually dispatched within 5-10 working days
    $ 587.00
    Catalog Numberorb345885
    CategoryAntibodies
    DescriptionTubulin alpha antibody (Biotin)
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, IF, IHC, WB
    ReactivityBovine, Gallus, Goat, Human, Mouse, Rat, Sheep
    IsotypeIgG
    ImmunogenAnti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
    Concentration0.61 mg/ml
    Dilution rangeELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, IF: 1:500 - 1:2,000, WB: 1:2,000-1:10,000
    Form/AppearanceLyophilized
    PurityAnti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein. The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
    ConjugationBiotin
    UniProt IDP68363
    StorageStore Anti-Tubulin Loading Control Antibody at 4° C prior to restoration. For extended storage aliquot Control Antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge Tubulin Antibody if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesrabbit anti-alpha-Tubulin Antibody biotin conjugat
    Read more...
    NoteFor research use only
    Application notesAnti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.
    Expiration Date12 months from date of receipt.
    Tubulin alpha antibody (Biotin)

    ELISA Results of Purified Rabbit Anti-Alpha Tubulin Antibody Biotin Conjugated tested against purified Alpha Tubulin Biotin Conjugated. Each well was coated in duplicate with 0.1 µg of BSA conjugated Alpha Tubulin. The working dilution is 1:11000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654), and TMB substrate (p/n orb348651).

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