alpha tubulin antibody (Biotin)

• Catalog Number: orb44530
• Category: Antibodies
• Description: Mouse Monoclonal to alpha tubulin.
• Clonality: Monoclonal
• Clone Number: TU-01
• Tested applications: FC, ICC, IHC-P, WB
• Reactivity: Aves, Human, Invertebrate, Mouse, Paramecium, Plant, Porcine, Yeast
• Isotype: Mouse IgG1
• Immunogen: Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: alpha-Tubulin
• Entrez: 7277
• UniProt ID: Q71U36
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-alpha-tubulin antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of human alpha-tubulin using mouse monoclonal antibody TU-01 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated streptavidin. A specific band was detected for alpha-tubulin at approximately 54 kDa.