Tuberin/TSC2 Antibody

• Catalog Number: orb196291
• Category: Antibodies
• Description: Tuberin/TSC2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Tuberin recombinant protein (Position: H1611-V1807). Human Tuberin shares 94% and 90% amino acid (aa) sequences identity with mouse and rat Tuberin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 200608 MW
• UniProt ID: P49815
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Tuberin;Tuberous sclerosis 2 protein;TSC2;TSC4;
• Note: For research use only
• Application notes: WB: The detection limit for Tuberin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Tuberin using anti-Tuberin antibody.Lane 1:recombinant human Tuberin protein 0.5 ng.

WB analysis of Tuberin using anti-Tuberin antibody.Lane 1:human U20S cell; 2:human PANC cell; 3:human HepG2 cell; 4:human A549 cell; 5:human COLO320 cell.

IHC analysis of Tuberin using anti-Tuberin antibody. Tuberin was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of Tuberin using anti-Tuberin antibody. Tuberin was detected in a paraffin-embedded section of rat brain tissue.

IHC analysis of Tuberin using anti-Tuberin antibody. Tuberin was detected in a paraffin-embedded section of human mammary cancer tissue.