TTF1/NKX2-1 Rabbit Polyclonal Antibody

• Catalog Number: orb186420
• Category: Antibodies
• Description: TTF1/NKX2-1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Gallus, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TTF1 (121-220/371aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 38 kDa
• Target: NKX2-1
• UniProt ID: P43699
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: NKX21_HUMAN; Homeobox protein Nkx-2.1; Homeobox pr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): RSC96 Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-TTF1/AF647 Conjugated antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.

Cell: A549, Concentration: 1:100, Host/Isotype: Rabbit/IgG (blue), Flow cytometric analysis of Rabbit IgG isotype control (Cat#: orb186420) on A549 (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

Sample: Lane 1: Mouse Thyroid gland tissue lysates, Primary: Anti-TTF1 (orb186420) at 1/200 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 38 kDa, Observed band size: 42 kDa.