TSPO/PBR Antibody

• Catalog Number: orb1538634
• Category: Antibodies
• Description: TSPO/PBR Antibody
• Target: TSPO / PBR
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-RDNSGRRGGSRLPE, from the C-Terminus of protein sequence according to NP_033905.3.
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:8000), WB (1 - 2 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx. 18kDa band observed in Mouse Spleen lysates (calculated MW of 18.8kDa according to NP_033905.3). Recommended concentration: 0.5-1 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TSPO, BZRP, BPBS, IBP, MBR, MDRC, PBR, PBRS, PBS,
• Note: For research use only

Anti-TSPO / PBR antibody IHC of human adipose. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

PBR (mouse) Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

TSPO antibody (0.5 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PBR (mouse) Antibody Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton.