Tspo Antibody

• Catalog Number: orb1247339
• Category: Antibodies
• Description: Tspo Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-RDNSGRRGGSRLPE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx.17+18kDa doublet band observed in cell lysates of NIH3T3 and in Rat Adrenal Gland lysates (calculated MW of 18.8kDa according to Mouse NP_033905.3 and 18.9kDa according to Rat NP_036647.1). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Tspo
• UniProt ID: P50637
• NCBI: NP_033905.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PBR, Tspo, translocator protein, Bzrp, IBP, benzod
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx.17+18kDa doublet band observed in cell lysates of NIH3T3 and in Rat Adrenal Gland lysates (calculated MW of 18.8kDa according to Mouse NP_033905.3 and 18.9kDa according to Rat NP_036647.1). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247339 (3.75 ug/ml) staining of paraffin embedded Human Renal Tubules. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247339 (3.75 ug/ml) staining of paraffin embedded Human Squamous Epithelium. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining. This data is from a previous batch, not on sale.

orb1247339 (3.75 ug/ml) staining of paraffin embedded Human Adipose. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247339 (2 ug/ml) staining of NIH3T3 (A) amd Rat Adrenal Gland (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247339 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing mitochondrial /cytoplasmic staining.

orb1247339 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1247339 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).