TSG101 Rabbit Polyclonal Antibody

• Catalog Number: orb11527
• Category: Antibodies
• Description: TSG101 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Rabbit
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Tsg101 (4-120/390aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 44 kDa
• Target: Tsg101
• UniProt ID: Q99816
• RRID: AB_10747693
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Tumor susceptibility gene 101 protein; ESCRT I com
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Lee, Jienny et al. Canine Natural Killer Cell-Derived Exosomes Exhibit Antitumor Activity in a Mouse Model of Canine Mammary Tumor Biomed Res Int, 2021, 6690704 (2021)

PubMed: 34527741

Sample: Lane 1: Brain (Rat) Lysate at 30 ug, Lane 2: Heart (Rat) Lysate at 30 ug, Primary: Anti-Tsg101 (orb11527) at 1:200 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000 dilution, Predicted band size: 43kD, Observed band size: 43kD.

Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-TSG101 (orb11527) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kD, Observed band size: 46 kD.

Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 2: 293T (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-Tsg101 (orb11527) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kD, Observed band size: 46 kD.