Trypsin antibody (Biotin)

• Catalog Number: orb344767
• Category: Antibodies
• Description: Trypsin antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Trypsin Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas]. Cross reactivity against Trypsin from other sources may occur but has not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P00760
• NCBI: CAA38513.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-trypsin antibody biotin conjugation, b
• Note: For research use only
• Application notes: Anti-Trypsin Biotin Antibody has been tested by ELISA and western blot. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.