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Catalog Number | orb344979 |
---|---|
Category | Antibodies |
Description | Trypsin Inhibitor antibody (Biotin) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Plant |
Isotype | IgG |
Immunogen | Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500 |
Form/Appearance | Lyophilized |
Purity | Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soy Bean]. Cross reactivity against Trypsin Inhibitor from other sources is unknown. |
Conjugation | Biotin |
UniProt ID | P01070 |
NCBI | P01070.2 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-trypsin inhibitor antibody biotin conj Read more... |
Note | For research use only |
Application notes | Anti-Trypsin Inhibitor Biotin Conjugated antibody has been tested by ELISA and western blotting. Researchers should determine optimal titers for applications that are not stated below. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of ~1 ug of purified Soybean Trypsin Inhibitor Reduced (Lane1), 0.25 ug of purified Soybean Trypsin Inhibitor Reduced (Lane2), ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced (Lane3), 0.25 ug of purified Soybean Trypsin Inhib
Western blot analysis of Lane 1: Soybean Trypsin Inhibitor Reduced. Lane 2: Soybean Trypsin Inhibitor Non-Reduced. Lane 3: Streptavidin Reduced. Lane 4: Streptavidin Non-reduced. Load: ~1 ug per lane using Trypsin Inhibitor antibody (Biotin)
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