TRP2 Rabbit Polyclonal Antibody

• Catalog Number: orb11521
• Category: Antibodies
• Description: TRP2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Mouse, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TRP2 (441-552/552aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: DCT
• UniProt ID: P40126
• RRID: AB_10746741
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: L dopachrome tautomerase; DCT; DCT; Dopachrome del
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Elkhamisy, Fatma Alzahraa A.a et al. Trophoblast cell surface antigen 2 expression in scalp basal cell carcinomas: does it differ from the facial basal cell carcinomas location? Egyptian Journal of, (2024)

PubMed:

Applications

IHC

Reactivity

Human

Chen, Minghan et al. Keratinocytes take part in the regulation of substance P in melanogenesis through the HPA axis J Dermatol Sci, (2022)

PubMed: 35525773

Paraformaldehyde-fixed, paraffin embedded (Human melanoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TRP2) Polyclonal Antibody, Unconjugated (orb11521) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.