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Catalog Number | orb1825396 |
---|---|
Category | Antibodies |
Description | The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, is mainly expressed in nervous tissue, and is a functional receptor for NT-3. Four forms of Trk C are produced by alternative splicing. Isoform A is full length Trk C, isoform B differs from A between amino acids 529 to 612 and is missing amino acids 613 to 839. Trk C, isoform C is missing amino acids 712 to 725 and isoform D is missing amino acids 402 to 410. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | NTRK3/7140 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | A recombinant partial protein sequence (within amino acids 100-300) from the human protein was used as the immunogen for the TrkC antibody. |
Antibody Type | Primary Antibody |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This TrkC antibody is available for research use only. |
UniProt ID | Q16288 |
Storage | Aliquot the TrkC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human breast cancer with TrkC antibody (clone NTRK3/7140). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with TrkC antibody (clone NTRK3/7140). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brain tissue with TrkC antibody (clone NTRK3/7140). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human salivary gland with TrkC antibody (clone NTRK3/7140). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free TrkC antibody (clone NTRK3/7140) as confirmation of integrity and purity.
IHC, WB | |
Bovine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Zebrafish | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Gallus | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |