TrkC Antibody

• Catalog Number: orb1825396
• Category: Antibodies
• Description: The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, is mainly expressed in nervous tissue, and is a functional receptor for NT-3. Four forms of Trk C are produced by alternative splicing. Isoform A is full length Trk C, isoform B differs from A between amino acids 529 to 612 and is missing amino acids 613 to 839. Trk C, isoform C is missing amino acids 712 to 725 and isoform D is missing amino acids 402 to 410.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant partial protein sequence (within amino acids 100-300) from the human protein was used as the immunogen for the TrkC antibody.
• UniProt ID: Q16288
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Antibody Type: Primary Antibody
• Clone Number: NTRK3/7140
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the TrkC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This TrkC antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human breast cancer with TrkC antibody (clone NTRK3/7140). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with TrkC antibody (clone NTRK3/7140). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human brain tissue with TrkC antibody (clone NTRK3/7140). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human salivary gland with TrkC antibody (clone NTRK3/7140). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free TrkC antibody (clone NTRK3/7140) as confirmation of integrity and purity.