TRIT1 Rabbit Polyclonal Antibody

SKU: orb186373

Description

TRIT1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TRIT1 (121-220/467aa)
• Target: TRIT1
• Molecular Weight: 48 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

2310075G14Rik; AI314189; AI314635; AV099619; FLJ20061; hGRO1; IPP transferase; IPPT; IPT; IPTase; Isopentenyl diphosphate:tRNA isopentenyltransferase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; MGC149242; MGC149243; mitochondrial; MOD5; MOD5_HUMAN; OTTMUSP00000009314; Putative protein TR2IT1; RP23-121J14.6; TRIT 1; TRIT1; tRNA dimethylallyltransferase; tRNA isopentenylpyrophosphate transferase; tRNA isopentenyltransferase 1; tRNA isopentenyltransferase; tRNA isopentenyltransferase mitochondrial.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-TRIT1 (orb186373) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 48kD, Observed band size: 53kD.

Sample: Uterus (Mouse) Lysate at 40 ug, Primary: Anti-TRIT1 (orb186373) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 48kD, Observed band size: 53kD.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-TRIT1 (orb186373) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 48kD, Observed band size: 53kD.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TRIT1) Polyclonal Antibody, Unconjugated (orb186373) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

TRIT1

UniProt

Q9H3H1