Triple FLAG peptide

• Catalog Number: orb343861
• Category: Proteins
• Description: Triple FLAG peptide
• Tested applications: IP, SDS-PAGE, WB
• Concentration: 1.0 mg/ml
• Dilution range: IP: YES - see PROTOCOL, WB: YES as blocking peptide in PCA
• Form/Appearance: Lyophilized
• Purity: Greater than 90% specific peptide by HPLC and MS.
• Conjugation: Unconjugated
• Storage: Store vial at -20 ° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: synthetic FLAG triple peptide, Met-Asp-Tyr-Lys-Asp
• Note: For research use only
• Application notes: The Triple FLAG Peptide has been tested by SDS-PAGE. A working concentration of 500 μg/mL for triple FLAG peptide is typically used for competitive elution of FLAG containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG affinity gel PROTOCOL for recommendations for use.
• Expiration Date: 12 months from date of receipt.