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Triple FLAG peptide
Catalog Number: orb343861
| Catalog Number | orb343861 |
|---|---|
| Category | Proteins |
| Description | Triple FLAG peptide |
| Tested applications | IP, SDS-PAGE, WB |
| Concentration | 1.0 mg/ml |
| Dilution range | IP: YES - see PROTOCOL, WB: YES as blocking peptide in PCA |
| Form/Appearance | Lyophilized |
| Purity | Greater than 90% specific peptide by HPLC and MS. |
| Conjugation | Unconjugated |
| Storage | Store vial at -20 ° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. |
| Buffer/Preservatives | None |
| Alternative names | synthetic FLAG triple peptide, Met-Asp-Tyr-Lys-Asp Read more... |
| Note | For research use only |
| Application notes | The Triple FLAG Peptide has been tested by SDS-PAGE. A working concentration of 500 μg/mL for triple FLAG peptide is typically used for competitive elution of FLAG containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG affinity gel PROTOCOL for recommendations for use. |
| Expiration Date | 12 months from date of receipt. |
SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel. Lane 1: DYKDDDDK tagged recombinant protein. Lane 2: flow through. Lane 3: wash. Lane 4: eluted fraction one. Lane 5: eluted fraction two. Lane 6: eluted fraction three. Lane 7: blank. Lane 8: molecular weight markers. Load: 5 µg protein lane 1, 15 µl per each other lane. Predicted/Observed size: 135kDa for DYKDDDDK tagged recombinant protein.
