TRIP1 Rabbit Polyclonal Antibody

SKU: orb100240

Description

TRIP1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Gallus, Human, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TRIP1/PSMC5 (122-320/406aa)
• Target: PSMC5
• Molecular Weight: 45 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

26S protease regulatory subunit 8; Cim3; MSUG1 protein; p45 antibody p45/SUG; Proteasome(prosome macropain) 26S subunit ATPase 5; Proteasome 26S ATPase subunit 5; Proteasome 26S subunit ATPase 5; Proteasome prosome macropain 26S subunit ATPase 5; Proteasome subunit p45; PSMC 5; PSMC5; Rpt6; S8 antibody SUG 1; SUG1; Tat binding protein homolog 10; TBP 10; TBP10; Thyroid hormone receptor interacting protein 1; Thyroid receptor interactor 1; TRIP 1; TRIP1; TRIP1(SUG1).

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TRIP1) Polyclonal Antibody, Unconjugated (orb100240) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TRIP1) Polyclonal Antibody, Unconjugated (orb100240) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-TRIP1 (orb100240) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 45 kD.

Quick Database Links

Gene Symbol

PSMC5

UniProt

P62195