Triosephosphate isomerase Rabbit Polyclonal Antibody

SKU: orb7113

Description

Triosephosphate isomerase Rabbit Polyclonal Antibody

Research Area

Cell Biology, Energy Metabolism, Metabolism

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Triosephosphate isomerase (151-249/249aa)
• Target: TPI1
• Molecular Weight: 27 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

TIM; TPI 1; TPI; TPI1; Triose phosphate isomerase 1; Triose phosphate isomerase; Triosephosphate isomerase 1; Triosephosphate isomerase; MGC88108; TPIS_HUMAN.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-Triosephosphate isomerase (orb7113) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Triosephosphate isomerasee (orb7113) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 30 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TPIS Polyclonal Antibody, Unconjugated (orb7113) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

TPI1

UniProt

P60174

References

Kwon, W.-S. et al. Comparison of markers predicting litter size in different pig breeds Andrology, 5, 568-577 (2017)

PubMed: 28409901