TRIM65 Rabbit Polyclonal Antibody

• Catalog Number: orb186360
• Category: Antibodies
• Description: TRIM65 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TRIM65 (301-400/517aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 57 kDa
• Target: TRIM65
• UniProt ID: Q6PJ69
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Tripartite motif containing protein 65; TRI65_HUMA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhi-Xiang Zhou 1, Xiao-Feng Ma 2, Wen-Hao Xiong 1, Zhong Ren 1, Miao Jiang 1, Nian-Hua Deng 1, Bo-Bin Zhou 1, Hui-Ting Liu 1, Kun Zhou 1, Heng-Jing Hu 3, Hui-Fang Tang 3, He Zheng 4, Zhi-Sheng Jiang TRIM65 promotes vascular smooth muscle cell phenotypic transformation by activating PI3K/Akt/mTOR signaling during atherogenesis Atherosclerosis, (2024)

PubMed: 38301602

Applications

WB

Reactivity

Human

Sample: Lane 1: Human HUVEC cell lysates, Lane 2: Human 293T cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-TRIM65 (orb186360) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kDa, Observed band size: 58 kDa.