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Item 1 of 4
TRIM65 Antibody (Center)
Catalog Number: orb1937931
| Catalog Number | orb1937931 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB29692 |
| Tested applications | FC, IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 57353 Da |
| Target | This TRIM65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-355 amino acids from the Central region of human TRIM65. |
| UniProt ID | Q6PJ69 |
| NCBI | NP_001243053.1, NP_775818.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Tripartite motif-containing protein 65, TRIM65 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Confocal immunofluorescent analysis of TRIM65 Antibody (Center) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
TRIM65 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TRIM65 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TRIM65 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
TRIM65 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the TRIM65 antibody detected the TRIM65 protein (arrow).
