TRIM55 antibody

• Catalog Number: orb18497
• Category: Antibodies
• Description: Goat polyclonal antibody to TRIM55
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 60.5; 50.8; 26.8
• Target: MURF2 / TRIM55
• Entrez: 84675
• Protein Sequence: HIFSFSWLNSLNE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TRIM55 antibody, anti RNF29 antibody, anti MU
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 85kDa band in human skeletal muscle lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 60.5kDa according to NP_908973.1. The 85kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Heart lysate using TRIM55 antibody

Immunohistochemical staining of Human Heart using TRIM55 antibody