TRIM55 Antibody

• Catalog Number: orb1247239
• Category: Antibodies
• Description: TRIM55 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HIFSFSWLNSLNE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 58kDa band observed in lysates of cell line HeLa (calculated MW of 60.5kDa according to NP_908973.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig, Rat and Mouse Heart, and Pig and Mouse Skeletal Muscle for this particular batch.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TRIM55
• UniProt ID: Q9BYV6
• NCBI: NP_908975.1, NP_908973.1, NP_908974.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TRIM55, RNF29, MURF-2, ring finger protein 29, for
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 58kDa band observed in lysates of cell line HeLa (calculated MW of 60.5kDa according to NP_908973.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig, Rat and Mouse Heart, and Pig and Mouse Skeletal Muscle for this particular batch.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247239 (2 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247239 (10 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247239 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi and cytoplasmic staining.

orb1247239 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).